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In order to study antibiotic activities of basidomycetes (mushroom), 68 species of mushroom were extracted with petroleum ether, 811% ethanol, and distilled water in that order. A total of 204 extracts were obtained. Antibiotic activities against Microsporum gypseum were observed from the petroleum ether extracts of Aborelporus DGU-L6 mushroom, and the water extracts of Clitogbe DGU-7 mushroom. Antibiotic activity against Aspergillus niger were observed from the 80% ethanol extracts of Cortinarius DGU-51 and Marasmminus DGU-L67 mushroom. The petroleum ether extracts of Hetero DGU-L25 mushroom showed various antibiotic activities, particularly strong activities against M. cams. and the minimum inhibitory concentration (MIC) was 40 ㎍/㎖. The extracts also showed antibiotic activities against A, niger (KCTC 2025), A. niger (KCTC 2118), A. versicobr (KCTC 2120), A. Jlavus (KCTC 2117), M. gypsem, Pyricularia oryzae, and Triehopyto mentagrophytes, and MIC for each fungus was 600 ㎍/㎖, 500 ㎍/㎖, 800 ㎍/㎖, 100 ㎍/㎖, 600 ㎍/㎖, 200 ㎍/㎖, and 600 ㎍/㎖, respectively.
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